Lab Protocols AutoclavingChanging CO2 tanks on incubatorsThawing mammalian cellsFreezing mammalian cellsHeat inactivation of FBSLysis buffer recipeLysing adherent mammalian cellsIP lysis buffer recipe Immunoprecipitation (IP) protocol Cell fractionation: isolation of nuclei BCA protein concentration assay Micro-BCA protein concentration assay Mini gel castingMidi gel casting, electrophoresis, and blottingSDS-PAGE recipe NuPAGE western blot protocol Trans-Blot Turbo semi dry blottingWet tank transfer protocolLentivirus preparationLentivirus transfection worksheet, 3rd generation packagingLentivirus transfection worksheet, 2nd generation packaging Lentivirus preparation (Fugene-assisted)Retrovirus preparation using Phoenix cells Retrovirus transfection worksheet Retrovirus preparation, VSVG pseudotyping Coverslip immunofluorescence Growing cell spheroids in collagenSoft agar colony formation assayWound healing assay Ethanol fixation for cell cycle analysis Media recipe for mouse PDAC cell lines Subculturing glioma stem cells (GSCs) Transforming competent bacterial cell lines Transforming bacteria with pSLIK vectorsMaking LB agar plates Growing bacterial cultures Concentrating DNA by precipitation in ethanol Reconstituting DNA from filter paper DNA gel electrophoresis qRT-PCR using SYBR GreenQiagen RNeasy Mini Handbook Eurofins sequencing Heatmap generation in MeV softwareR Studio code for PCA, Pearson, and heatmap workflow